Molecular Cloning of Three Genes Encoding G Protein Alpha Subunits in the White Root Rot Fungus, Rosellinia necatrix

NoteMolecular Cloning of Three Genes Encoding G Protein in the White Root Rot Fungus , Rosentnia necatrix JSBA Alpha Subunits

is very diMcult to prevent, has done great damage to comrnercially grown grape vines and apple and pear trees, among others, Unfertunately, despite its importance, little is known about the molecular or cellular biology of this fungus. This study is a part of our attempt te better understand the signal transduction pathways mediating the vegetative incompatibility of Rosellinia. In order to iso...

Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete

Characterization of the Rosellinia necatrix Transcriptome and Genes Related to Pathogenesis by Single-Molecule mRNA Sequencing

White root rot disease, caused by the pathogen Rosellinia necatrix, is one of the world's most devastating plant fungal diseases and affects several commercially important species of fruit trees and crops. Recent global outbreaks of R. necatrix and advances in molecular techniques have both increased interest in this pathogen. However, the lack of information regarding the genomic structure and...

Cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus Phanerochaete sordida YK-624.

Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-...

G alpha 12 and G alpha 13 subunits define a fourth class of G protein alpha subunits.

Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) are central to the signaling processes of multicellular organisms. We have explored the diversity of the G protein subunits in mammals and found evidence for a large family of genes that encode the alpha subunits. Amino acid sequence comparisons show that the different alpha subunits fall into at least three classes. The...